#4513: Plate Reader: A multi-detection microplate reader configured to measure the output of the UDP tracer is required. 8 foot) 4400-0002 Power cord, 1 meter (3. SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax. SpectraMax fluorescence microplate reader with SoftMax® Pro Software, is a quick, sensitive detection method for proteins. You should determine the expected mean OD value by reading a clean water-filled plate from each new lot. Plate reader set to 50 reads. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR. After washing with PBS, the fluorescence intensities for each staining were analyzed with a fluorescence plate reader (SpectraMax M2). SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). A whole well fluorescence imaging system that can collect information from all cells inside the well, from 6- to 384-well formats. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Absorbance; Fluorescence (FI, TR, FP) Luminescence. The instrument is located in room 2131 at 300 George Street. Luminex Magpix. Fluorescence Microplate Reader Comparison Chart. Figure 2. Last lab had ours for years and never had a problem with it. Features. When a feature or capability applies to only certain readers, this exception is noted. 305 reference level saturation. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single Fig 2: Enzymatic response of synthetic compounds. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Type. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr:Reads a 96-well plate in 15 s, a 384-well plate in. 0. In the lower half of the window, left side, there is a box labeled reader. . plate over and over again. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Fluorescence is measured at time zero and again after one-hour of incubation at 37°C in a fluorescence microplate reader. In the M2, M5 and Plus 384, the light. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. The SPECTRAmax GEMINI XS Microplate Spectrofluorometer from Molecular Devices is a microplate reader capable of detecting signal from either fluorescence or luminescence based assays. Molecular Devices SpectraMax M2. 304 signal level saturation. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. The excitation and the emission. Explore how the Gemini EM Microplate Reader is a flexible spectrofluorometer capable of running many fluorescence-based assays using its dual. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Whether you need to measure fluorescence, absorbance, luminescence, or time-resolved fluorescence (TRF), or you use PerkinElmer ™ AlphaScreen. S. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Get unrivalled sensitivity and reproducibility and elevate your microplate assay protocol with the Eppendorf Assay/Reader Plates. flexibility of monochromator-based systems. To avoid these issues and provide the best possible dynamic range between the highest and the lowest measurement values of your assay, fluorescence gain is typically adjusted on the sample with the expected highest signal output (e. For a higher . Optimized for fluorescence microplate readers. Optimized Settings to Validate the Molecular Devices SpectraMax® M2/M2e Microplate Readers with the Transcreener® Fluorescence Intensity Assays. 5. SoftMax Pro. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time. Published Dec 16, 2020 | Updated Dec 16, 2020. Plate Shaking Linear, orbital, double-orbital Dimensions (H x W x D) 12 x 15 x 15 in. Microplate Readers Include the M3, M4, M5, and M5. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. 00. The cuvette must be in place when you read the plate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. When a. spr SpectraMax iD5 SpectraTest ABS2 v1. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. The plate is set up for reading and images are acquired according to specified parameters. Downloads. Read More. Description 1. A solution for low volume applications. It features a modular design that allows users to add or change detection cartridges according to their needs. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Other features and benefits include: Eliminate changing out filters. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® Assays. Cells in each image are identified by SoftMax Pro Software and cell-by-cellfluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. Auto-calibration and self-diagnostics. Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. It also. These lab workhorses are used in drug discovery. E Models. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. Based on the SpectraMax M5e platform, this five-mode reader addresses multiple application technologies including: absorbance, fluorescenceSeller assumes all responsibility for this listing. 0077 OD 0. Linear, Orbital Slow, and Orbital Fast. As new assays are developed or yourFor TRF assays, you will need a fluorescence plate reader equipped with a time-resolved fluorometry option and a 330 nm excitation filter and emission filters capable of detecting 647 nm (620/40) and 665 nm (665/ 7. Qualify the fluorescence performance of the SpectraMax . SpectraMax M2 and use thereof is covered by issued (U. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. Stage and carriage are homed and position is tested and verified. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 020 OD 2. Optical System: High energy xenon flash lamp. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Add to Cart. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax ® Pro. Molecular Devices SpectraMax M2 Multimode Plate Reader. For fluorescent assays, black plates absorb light and reduce background and crosstalk. S. 40266 Email: drugdiscoverytech@lifetech. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. SoftMax Pro Software has been integrated by many leading robotics and LIMS partners, enabling both data analysis and. Calcein AM is a widely used live-cell marker. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Luminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. FDSS µCell is a whole plate imager that measures fluorescence or luminescence from all wells of microplate continuously and simultaneously with built-in 96/384 channel liquid handling robotics. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Tristar 3 Multimode Microplate Reader. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard plate reader applications, protecting your initial investment. The plate reader offers a fast, simple readout of average fluorescence per well, while the cytometer gives more detailed information, including total cell number and. 53 - 60 kg, (117-132 lb), depending on configuration. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideELISA Plate Readers / Microplate Readers. Fig 2: Enzymatic response of synthetic compounds. g. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance. Many of the following instruments are multimode readers that, in addition to luminescence detection, can also be used to measure UV. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Molecular Devices SpectraMax™ M2e Microplate Reader w/ Warranty. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. Endpoint, kinetic, spectrum. When connecting for the first time to either the SpectraMax L reader or StakMax system. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 00 USD. 8 is required to operate the new SpectraMax® M5 reader and its five read types: Fluorescence Intensity, Absorbance, Luminescence, Time-Resolved Fluorescence (TRF) and Fluorescence Polarization. The first step consisted of taking an initial fluorescence measurement using Molecular Devices SpectraMax® M2 fluorescence plate reader of a 96-well plate containing PET-RAFT polymerization reaction mixtures in individual wells. Add to Cart. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. 0) OD (0. Transcreener ® ADP. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 2018. Analyze samples using fluorometry, photometry, luminometry, TRF or FP with these. Detection Ranges: absorbance 200-1000nm, fluorescence Ex: 250-850nm / fluorescence Em: 360-850nm, luminescence: 360–850 nm. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. A decrease in fluorescence with increasing staurosporine concentration was detected with the SpectraMax iD3 reader. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader Absorbance ü ü ü ü Fluorescence Intensity ü ü ü ü Luminescence ü ü ü ü Time-Resolved Fluorescence ü ü ü Fluorescence Polarization ü ü HTRF ü Upgrade Options TRF, HTRF, FP HTRF, FP HTRF N/A Plate Formats 6-, 12-, 24-, 48-, 96-, 384. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190. I am using the filter pair of 480/535 and trying to measure fluorescence. $ 2,499. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Top detection is available for fluorescence detection on the SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2e. The plate blank’s RFU values should be subtracted from the RFUs of all samples of the plate. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader. spr SpectraMax Plus 384. D 3 1. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. By employing dyes that specifically bind to nucleic acids, DNA/RNA concentrations can also be quantified using fluorescence detection. 58% for the detection module and 2. Analyze samples using fluorometry, photometry, luminometry, TRF or FP with these accurate, versatile microplate readers. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. fluorescence intensity (Figure 6). Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Typical settings for SpectraMax readers with fluorescence detection mode are shown. Fluorescence intensity values increased over time, but similar EC 50 values of 263 nM and 295 nM were obtained for assay incubation times ranging from one. SpectraMax M2 and use thereof is covered by issued (U. On the SpectraMax i3x reader, HTRF (cartridge) should be selected as the Optical Configuration. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single 1) Select the correct plate and the wells that you want to measure. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. 5 pM typical (0. 49 µM,. Add to Cart. 5°C at 37°C. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Temperature Control: ambient from +4 to 45C. 0 × 107) RLU 0. 2. Note: In this user guide, SpectraMax ® refers to several SpectraMax . spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. M2. NOTE: This assay differs from the previous one in that the fluorescent dye ThT or ThS is included in the aggregation reaction. Absorbance (monochromator). Therefore, ADP production is proportional to an increase in fluorescence. The SpectraMax® i3 Multi-Mode Detection Platform for absorbance, fluorescence, and luminescence detection, and the ability to add cartridges as new assays are developed. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Download PDF. A microplate reader as individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). On microplate readers,. Transcreener ® ADP. Fig 2: Enzymatic response of synthetic compounds. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. 209, but in 96-well plate was 0. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Then the working solution (50μL) was added to the plate for 30 min treatment without light at room temperature and then the stop solution (50μL) was added. Item Description Sensitivity 10amol/wellalkalinephosphate200µL Wavelengthrange 250-850nm Crosstalk <0. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. The GloMax® line includes high-performance plate readers with a range of detection options. item 1 Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O $4,999. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). TRF as Secondary Detection Mode: TRF is supported as a secondary detection mode by these readers: Gemini XS, EM, XPS, M2, & FlexStation. Multimode Readers Comparison Chart. 306 plate air cal fail,low light: 316 grating motor driver faulty. Additionally, all BMG LABTECH microplate readers offer the possibility to measure in very small volumes, down to 2 µl of the sample by using the LVis Plate. This enables the standard curve to be plotted and sample concentrations to be calculated automatically by SoftMax. Python 3. Self Service Available. M2. BioTek Synergy HTX Microplate Reader - Multi Mode (Abs + FL + Lum), Bio-tek, Used plate reader, BioTek multi-mode detection, PMT for luminescence detection, kinetic assays, Time Resolved Fluorescence, Gen5 software, absorbance reader, fluorescence detection, used lab. If a lab requires instruments to read ELISA plates, one to do fluorescence and one for luminescence, it will likely look for one instrument that can handle all these. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Plate reader. Usage/Application: Lab. STAKMAX. Listing: #4772697. 5%in96-and384-wellmicroplates. Molecular Devices SpectraMax M2 Microplate Reader. Microplate Formats: 6, 12, 24, 48, 96, 384. 2. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. read matrix, spectral scanning and detection of plate formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. 9000-0756. the reader’s stated range. Typical settings forTo maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. SpectraMax Quant dsDNA Assay Kits. 7. Perform the following steps to install the drivers: Start > Control Panel > System. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. If it is something different, click on the arrow to bring up a list of. 3) At the bottom of the strip, click on "Show. 7. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. Three data types for FP read types are also being introduced with v4. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideReader Documentation Version No. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. Perform the scan and note the wavelength that gives maximum emission. the reader’s stated range. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Learn how you can utilize microplate readers to drive landmark discoveries. SpectraMax Spectrofluorometers. Endpoint, kinetic, spectrum and area-well. 2. Published Dec 16, 2020 | Updated Dec 16, 2020. Molecular Devices SpectraMax M2 ; Molecular Devices. The chamber contains. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. When a. 3) At the bottom of the strip, click on "Show. Add to Cart. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. WinKQCL ® Software can be used with a compatible microplate reader to run the template and. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. A microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2. the reader’s stated range. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Plate Readers. 67. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. Next add 100 µl of workingThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Add to Cart. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. In the M2, M5 and Plus. Allows you to choose your sample format depending on your unique research and throughput needs. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. SpectraMax M2 Readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). We therefore gathered salient. The drawing shows a standard microplate of 15 mm height covered with a lid. Enables accurate fluorescence data with every run. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. 2 only) SpectraMax M-series Microplate readers. Dose-dependent enzyme essay Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemFAST AND ACCURATE METERTECH Microplate Reader $3,650. SpectraMax M2E Multimode plate reader with cuvette option, ABS and FLR reading and extended range. High tolerance for contaminants. Featuring temperature control up toThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Bottom Monochromator: Fluorescein 2. Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. Model: SpectraMax M2. 25 to 3. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. 00 CAD. 0 out of 5 $ 2,999. When the user applies a set temperature of 37°C, the top is heated to 37. A. (12. WinKQCL ® Software guides the user through the process of setting up a microplate template and running an assay to determine the amount of endotoxin in a sample solution. A solution for low volume applications. Offer Ends In: 16 Days 14: 39: 14. It is typically used to determine the concentration of nucleic acids and proteins. Skip to main content. , a positive control). A. PC software (SkanIt software) Dimensions (D x W x H) 58 x 53 x 51 cm (23 x 21 x 20 in) Weight. D 59 A. . Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Compact and tunable absorbance microplate readers DATA SHEET. Appendix A. Selecting your. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 0 × 103 to 2. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. 3. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633 (18) SARS-CoV-2 antibody signatures robustly predict diverse antiviral functions relevant for convalescent plasma therapy medRxiv September 16, 2022 Harini Natarajan. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization. (in addition to fluorescence imaging) on the SpectraMax i3x Multi-Mode Microplate Reader. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. For example, microplates containing different samples can be read using the Tecan Infinite® M1000 Pro Plate Reader. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. Microplate Reader Shelf. SpectraMax® M2 SpectraMax® M2e Multi-Mode Microplate Readers User Guide 0112-0102 E October 2018 SpectraMax M2 and M2e Microplate Reader User. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (plate reader can measure multiple readouts, including UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. An approach to gain setting that I like to use is to set the gain based on the sample with the most fluorescence in order to get about 30-50% of the maximal signal before the detector saturates. 00. Product Information. Microplate Readers. SpectraMax® Microplate Readers and SoftMax® Pro Software. 1 SoftMax Pro v4. Dose-dependent enzyme essayVisualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. 100 readings per well gives optimal Z’ values for this assay. 0 out of 5 $ 3,249. The method is based on. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. olecular saka. View online or download Molecular devices SpectraMax M3 User Manual. When using the PyroWave XM Reader, excitation wavelengths of 380 nm and emission wavelengths of 440 nm are used to measure fluorescence. 306 plate air cal fail,low light. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. Perkin Elmer Envision. the reader’s stated range. 67. Basic "FL Plate Reader start up protocol" (print and bring along for the. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Plate Shaking. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 303 unable to cal dark current. The kit includes everything you need: reagents, buffers and a microplate. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3. 1) A maximal fluorescence intensity of 177 ± 3 RFU was observed at the highest cell concentration (Table 1). The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. . Absorbance (monochromator) Fluorescence (monochromator; FI, TR, FP) Luminescence; Molecular Devices Spectramax M2. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. The Spectr. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. The drawing shows a standard microplate of 15 mm height covered with a lid. Fully tested with validations and warranty. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Molecular Devices i3x. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 0 out of 5 $ 9,995. 1 cm) Weight 27 lb. Also refer to this Multimode Reader Comparison Chart. Multimode microplate readers deliver both performance and economy. Double-click the unknown device with the yellow warning icon. 47 and 0. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SpectraMax M2 Readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. It can measure absorbance from 230 -1000 nm. Add to Cart. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. 67. With-dye, Continual Mode Assay on a SpectraMax M2 Plate Reader.